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Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish

Analysis of Liver Microenvironment During Early Progression of Non-Alcoholic Fatty Liver Disease-Associated Hepatocellular Carcinoma in Zebrafish

DOI:
10.3791/62457-v

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09:27 min

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April 01, 2021

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, ,
1Department of Developmental and Molecular Biology,Albert Einstein College of Medicine, 2Department of Medicine (Hepatology),Albert Einstein College of Medicine, 3Einstein-Mount Sinai Diabetes Research Center,Albert Einstein College of Medicine, 4Marion Bessin Liver Research Center,Albert Einstein College of Medicine

Chapters

  • 00:05Introduction
  • 01:08Preparation of 10% Cholesterol-Enriched Diet for Acute Cholesterol Surplus
  • 02:29NASH Induction with Short-Term Larvae Feeding with Cholesterol-Enriched Diet Static Conditions
  • 04:22Collection of 13 Days Post-Fertilization Larvae from Feeding Boxes
  • 05:06Non-Invasive Confocal Imaging Using the Zebrafish Wounding and Entrapment Device for Growth and Imaging (zWEDGI)
  • 05:54Immune Cell Recruitment Analysis
  • 06:49Results: Monitoring Liver Disease Progression in the Hepatocellular Carcinoma (HCC) Zebrafish Model
  • 08:30Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present how to generate a non-alcoholic fatty liver disease (NAFLD)-associated Hepatocellular Carcinoma (HCC) zebrafish model to study the impact of cholesterol surplus on liver microenvironment and immune cell landscape.

Tags

Keywords: ZebrafishLiverNon-alcoholic Fatty Liver DiseaseHepatocellular CarcinomaIntravital MicroscopyDiet-inducedMicroenvironmentImmune Cell InfiltrationMetabolic SyndromeCardiovascular DiseasesHigh Cholesterol DietDiethyl Ether

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