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How to Calculate and Validate Inter-brain Synchronization in a fNIRS Hyperscanning Study

How to Calculate and Validate Inter-brain Synchronization in a fNIRS Hyperscanning Study

Article DOI: 10.3791/62801-v 05:33 min September 8th, 2021
September 8th, 2021



The dynamics between coupled brains of individuals have been increasingly represented by inter-brain synchronization (IBS) when they coordinate with each other, mostly using simultaneous-recording signals of brains (namely hyperscanning) with fNIRS. In fNIRS hyperscanning studies, IBS has been commonly assessed through the wavelet transform coherence (WTC) method because of its advantage on expanding time series into time-frequency space where oscillations can be seen in a highly intuitive way. The observed IBS can be further validated via the permutation-based random pairing of the trial, partner, and condition. Here, a protocol is presented to describe how to obtain brain signals via fNIRS technology, calculate IBS through the WTC method, and validate IBS by permutation in a hyperscanning study. Further, we discuss the critical issues when using the above methods, including the choice of fNIRS signals, methods of data preprocessing, and optional parameters of computations. In summary, using the WTC method and permutation is a potentially standard pipeline for analyzing IBS in fNIRS hyperscanning studies, contributing to both the reproducibility and reliability of IBS.


Inter-brain Synchronization FNIRS Hyperscanning Study Calculate Validate Protocol Analysis Pipeline Coherent Method Permutation-based Chest Conditions Participants Data Pre-processing Principle Component Analysis Correlation-based Signal Improvement Method NPCA Filter Head Motion Artifacts HMR Motion Correct Underscore CBSI Wavelet Transform Coherent Function Cross Wavelet And Wavelet Coherent Toolbox Default Parameters Morlet Mother Wavelet Time Series Frequency Domain Continuous Wavelet Transformation Frequency Band
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