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Biochemistry
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测量来自母猪后代的先前冷冻心脏组织中的线粒体电子转移复合物:评估运动诱导的线粒体生物能量变化的模型
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Biochemistry
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JoVE Journal
Biochemistry
Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow: A Model to Assess Exercise-Induced Mitochondrial Bioenergetics Changes
Please note that all translations are automatically generated.
Click here for the English version.
测量来自母猪后代的先前冷冻心脏组织中的线粒体电子转移复合物:评估运动诱导的线粒体生物能量变化的模型
DOI:
10.3791/62809-v
•
08:04 min
•
August 16, 2021
•
Daniel Barrera
,
Sierra Upton
,
Megan Rauch
,
Tara Notarianni
,
Ki Suk Eum
,
Megan Liberty
,
Sarmila Venkoba Sah
,
Robert Liu
,
Sean Newcomer
,
Linda E. May
,
Emre Agbas
,
Jessica Sage
,
Edina Kosa
,
Abdulbaki Agbas
13
1
AdventHelath
,
2
University of Illinois Chicago School of Public Health
,
3
Lincoln Memorial University
,
4
Children’s Mercy Hospital
,
5
Scripps Clinic
,
6
Cincinnati Children’s Hospital Medical Center
,
7
Kansas City University
,
8
Roblex Rex Veterans Affairs Medical Center
,
9
California State University San Marcos
,
10
East Carolina University
,
11
Massachusetts Institute of Technology
,
12
Boehringer Ingelheim Norway KS
,
13
Heartland Center for Mitochondrial Medicine
Chapters
00:05
Introduction
01:12
Mitochondria Isolation from Frozen Heart Tissue
06:18
Results: Exercise-Induced Changes in Mitochondrial ETC and Supercomplex Formation
07:27
Conclusion
Summary
Automatic Translation
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Automatic Translation
从先前冷冻的存档固体组织中制备富含线粒体的样品,使研究人员能够以各种实验方式对线粒体进行功能和分析评估。这项研究展示了如何从冷冻的心脏组织制备富含线粒体的制剂,并对线粒体进行分析评估。
Tags
Mitochondrial Electron Transfer
Sow Offspring
Exercise-induced Mitochondrial Bioenergetics
Mitochondria Preparation
Mitochondrial Yield
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