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Measuring Glucose Uptake in Drosophila Models of TDP-43 Proteinopathy
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JoVE Journal Neuroscience
Measuring Glucose Uptake in Drosophila Models of TDP-43 Proteinopathy

Measuring Glucose Uptake in Drosophila Models of TDP-43 Proteinopathy

DOI:
10.3791/62936-v

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07:07 min

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August 03, 2021

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, , ,
1Department of Molecular and Cellular Biology,University of Arizona, Tucson, 2Vollum Institute,Oregon Health and Science University, 3Department of Neuroscience,University of Arizona, Tucson

Chapters

  • 00:04Introduction
  • 00:41Drosophila Ventral Nerve Cord Dissections
  • 01:43Image Acquisition
  • 03:27Image Processing, ROI Selection, and Data Analysis
  • 04:46Results: Glucose Uptake Measurement in Drosophila Models of TDP-43 Proteinopathy
  • 06:32Conclusion

Summary

Automatic Translation

Automatic Translation

Glucose uptake is increased in Drosophila motor neurons affected by TAR DNA binding protein (TDP-43) proteinopathy, as indicated by a FRET-based, genetically encoded glucose sensor.

Tags

Keywords: Glucose UptakeDrosophilaTDP-43 ProteinopathyFRET-based SensorMotor NeuronsALS ModelsLive ImagingCentral Nervous SystemVentral Nerve CordConfocal Microscopy

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