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Ein ertragreiches Streptomyces-Transkriptions-Translation-Toolkit für synthetische Biologie und Naturstoffanwendungen
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Ein ertragreiches Streptomyces-Transkriptions-Translation-Toolkit für synthetische Biologie und Naturstoffanwendungen

Article DOI: 10.3791/63012-v • 07:59 min • September 10th, 2021
September 10th, 2021 •
1,2,3,4, 5, 5, 1,2,3,4,6,7, 5
1Centre for Synthetic Biology and Innovation, South Kensington Campus, 2Department of Medicine, South Kensington Campus, 3Section of Structural and Synthetic Biology, Department of Infectious Disease, Imperial College London, 4Sir Alexander Fleming Building, South Kensington Campus, 5School of Biosciences, Division of Natural Sciences, University of Kent, 6UK Dementia Research Institute Care Research and Technology Centre, Imperial College London; Hammersmith Campus, 7UK Innovation and Knowledge Centre for Synthetic Biology (SynbiCITE) and the London Biofoundry, Imperial College Translation & Innovation Hub

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Dieses Protokoll beschreibt eine verbesserte Methode zur Synthese hoher Ausbeuten rekombinanter Proteine aus einem zellfreien Transkriptionstranslationssystem (TX-TL) von Streptomyces venezuelae .

Tags

Bioengineering Cell-free protein synthesis in vitro transcription-translation Streptomyces Synthetic biology Systems biology cell-free systems biosynthesis
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