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Journal
/
Bioengineering
/
用于合成生物学和天然产物应用的高产
链霉菌
转录翻译工具包
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
A High-Yield
Streptomyces
Transcription-Translation Toolkit for Synthetic Biology and Natural Product Applications
Please note that all translations are automatically generated.
Click here for the English version.
用于合成生物学和天然产物应用的高产
链霉菌
转录翻译工具包
DOI:
10.3791/63012-v
•
07:59 min
•
September 10, 2021
•
Ming Toh
2,3,4
,
Kameshwari Chengan
,
Tanith Hanson
,
Paul S. Freemont
2,3,4,6,7
,
Simon J. Moore
1
Centre for Synthetic Biology and Innovation
,
South Kensington Campus
,
2
Department of Medicine
,
South Kensington Campus
,
3
Section of Structural and Synthetic Biology, Department of Infectious Disease
,
Imperial College London
,
4
Sir Alexander Fleming Building
,
South Kensington Campus
,
5
School of Biosciences, Division of Natural Sciences
,
University of Kent
,
6
UK Dementia Research Institute Care Research and Technology Centre
,
Imperial College London; Hammersmith Campus
,
7
UK Innovation and Knowledge Centre for Synthetic Biology (SynbiCITE) and the London Biofoundry
,
Imperial College Translation & Innovation Hub
Chapters
00:05
Introduction
00:58
Preparation of
Streptomyces venezuelae
ATCC 10712 Cells
03:56
Cell Lysis by Sonication
04:47
Cell Extract Clarification and Run-off Reaction
05:14
Set-up a Standard
Streptomyces venezuelae
Transcription-Translation (TX-TL) Reaction
06:21
Results:
Streptomyces
Transcription Translation Toolkit with the pTU1-A-SP44-mScarlet-I Plasmid
07:18
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议详细介绍了从
委内瑞拉链霉菌
无细胞转录翻译(TX-TL)系统合成高产量重组蛋白的增强方法。
Tags
Streptomyces Venezuelae
Cell-free Transcription-translation
High GC Content
Metabolic Enzymes
Biosynthetic Pathways
Heterologous Expression
S30A Buffer
S30B Buffer
Dithiothreitol (DTT)
Sonication
Crude Extracts
Non-model Organism
Synthetic Biology
Natural Product Applications
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