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The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination

Article DOI: 10.3791/63088-v • 04:50 min • February 8th, 2022
February 8th, 2022 •
1,2, 3,4, 3,5, 1,2,6, 7
1School of Chemistry and Molecular Biosciences, The University of Queensland, 2Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, 3Jiangsu Provincial Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, 4Department of Pharmaceutical Analysis, School of Pharmacy, Xuzhou Medical University, 5Department of Bioinformatics, School of Medical Informatics and Engineering, Xuzhou Medical University, 6Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture, Yangzhou University, 7Glycation and Diabetes Group, Mater Research Institute-The University of Queensland, Translational Research Institute

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Summary
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An optimal sucrose concentration was determined for the extraction of liver glycogen using sucrose density gradient centrifugation. The addition of a 10 min boiling step to inhibit glycogen-degrading enzymes proved beneficial.

Tags

Glycogen Structure Determination Glucose Polymer Blood Sugar Levels Properties Of Glycogen Extraction Method Sucrose Density Gradient Centrifugation Molecular Damage Sucrose Solution Density Glycogen Particles Sizes Boiling Step Denature Glycogen Degrading Enzymes
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