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Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
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Biochemistry
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JoVE Journal Biochemistry
Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

DOI:
10.3791/64367-v

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08:11 min

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August 09, 2022

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, ,
1Department of Chemistry and Henry Eyring Center for Cell & Genome Science,University of Utah

Chapters

  • 00:04Introduction
  • 00:42In Vitro Plate Reader Kinetics Assay
  • 04:03In Vivo Flow Cytometry Kinetics Assay
  • 06:27Results: Measurement of Fluorogenic RNA Aptamer Kinetics
  • 07:46Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol presents two methods to determine the kinetics of the fluorogenic RNA aptamers Spinach2 and Broccoli. The first method describes how to measure fluorogenic aptamer kinetics in vitro with a plate reader, while the second method details the measurement of fluorogenic aptamer kinetics in cells by flow cytometry.

Tags

Keywords: Fluorogenic RNA AptamersIn Vitro KineticsCellular KineticsReal-time StudiesRNA RenaturationThermocyclerPlate Reader InjectorFluorescence MeasurementsBinding BufferD-FHBI

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