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Biochemistry

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Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
 

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

Article DOI: 10.3791/64367-v
August 9th, 2022

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Summary August 9th, 2022

The protocol presents two methods to determine the kinetics of the fluorogenic RNA aptamers Spinach2 and Broccoli. The first method describes how to measure fluorogenic aptamer kinetics in vitro with a plate reader, while the second method details the measurement of fluorogenic aptamer kinetics in cells by flow cytometry.

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