/

/

从水生栖息地分离、繁殖和鉴定具有碳氢化合物代谢特性的细菌种类

JoVE Journal
Environment

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Environment

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats

Please note that all translations are automatically generated. Click here for the English version.

从水生栖息地分离、繁殖和鉴定具有碳氢化合物代谢特性的细菌种类

DOI:

10.3791/63101-v

•

10:03 min

•

December 07, 2021

•

Deepa Sethi, Richa Priyadarshini

¹Department of Life Sciences, School of Natural Sciences,Shiv Nadar University

Chapters

00:04Introduction
00:52Sample Collection, Processing, and Analysis
03:12Degradation of Hydrocarbons
05:04Genomic DNA Isolation of the Pure Culture
06:4916S rRNA Sequencing
08:07Results: Isolation and Characterization of Hydrocarbon Degrading Bacteria from Aquatic Habitats
09:11Conclusion

Summary

Automatic Translation

我们介绍了从水生栖息地分离，繁殖和表征碳氢化合物降解细菌的过程。该协议概述了细菌分离，通过16S rRNA方法鉴定以及测试其碳氢化合物降解潜力。本文将帮助研究人员表征环境样本中的微生物生物多样性，特别是筛选具有生物修复潜力的微生物。

Tags

Keywords: Hydrocarbon-degrading Microorganisms Aquatic Habitats Isolation Cultivation Identification Bacterial Species Water Sample Nutrient Media Dilution Colony Screening Gram Staining Glycerol Stocks Hydrocarbon Metabolism

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Helminth Collection and Identification from Wildlife

从个人污秽苍蝇检测食源性细菌病原体

一种优化富集技术的隔离<em>节杆菌</em>噬菌体种从土壤样品隔离

表征和被动采样的应用在农药水监测

实验协议生产生物柴油与饱和酮的分离从商业余积<em>等鞭金藻</em>藻类生物量

从鲟鱼去除耳石替代方法

Aquafaba 的成分和性质: 从商用罐装鹰嘴中回收的水

高效液相色谱-ESI-Q-MS 与红霉素通过光致降解法鉴定水生环境中的药物

流式细胞仪对水生生物膜的表征

高悬浮泥沙荷载和大型漂浮碎屑水生环境中 Microplastics 的取样、分类和表征

Read Article