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Detection of Foodborne Bacterial Pathogens from Individual Filth Flies
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JoVE Journal Environment
Detection of Foodborne Bacterial Pathogens from Individual Filth Flies

Detection of Foodborne Bacterial Pathogens from Individual Filth Flies

DOI:
10.3791/52372-v

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12:54 min

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February 13, 2015

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, , ,
1Center for Food Safety and Applied Nutrition,U.S. Food and Drug Administration

Chapters

  • 00:05Title
  • 02:36Fly Dissection and Bacterial Enrichment
  • 06:12Perform Lysis for the Detection of Salmonella and Cronobacter
  • 07:27PCR Detection of the Target Foodborne Pathogen
  • 08:30Isolation of Bacteria from PCR-positive Results
  • 10:38Results: Prevalence of Foodborne Pathogens in Wild Flies
  • 11:51Conclusion

Summary

Automatic Translation

Automatic Translation

A PCR-based protocol was adapted to detect Cronobacter spp., Salmonella enterica, and Listeria monocytogenes from body surfaces and alimentary canals of individual wild-caught flies. The goal of this protocol is to detect and isolate bacterial pathogens from individual insects collected as part of an environmental sampling program during foodborne outbreak investigations.

Tags

Keywords: Foodborne PathogensFilth FliesCronobacter Spp.Salmonella EntericaListeria MonocytogenesPCR-based DetectionEnvironmental SamplingFoodborne Outbreak InvestigationsInsect Vectors

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