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JoVE Journal
Environment

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Detection of Foodborne Bacterial Pathogens from Individual Filth Flies
 

Detection of Foodborne Bacterial Pathogens from Individual Filth Flies

Article doi: 10.3791/52372
February 13th, 2015

Summary February 13th, 2015

A PCR-based protocol was adapted to detect Cronobacter spp., Salmonella enterica, and Listeria monocytogenes from body surfaces and alimentary canals of individual wild-caught flies. The goal of this protocol is to detect and isolate bacterial pathogens from individual insects collected as part of an environmental sampling program during foodborne outbreak investigations.

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