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Biochemistry
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CD光谱学研究DNA-蛋白质相互作用
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Biochemistry
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JoVE Journal
Biochemistry
CD Spectroscopy to Study DNA-Protein Interactions
Please note that all translations are automatically generated.
Click here for the English version.
CD光谱学研究DNA-蛋白质相互作用
DOI:
10.3791/63147-v
•
06:48 min
•
February 10, 2022
•
Vijendra Arya
,
Anindita Dutta
,
Rohini Muthuswami
1
Jawaharlal Nehru University New Delhi
Chapters
00:04
Introduction
00:36
ATPase Activity
01:35
Preparation of Circular Dichroism (CD) Cuvettes and DNA Oligonucleotides
02:19
Setting up the Experiments and Recording the Scan
04:10
Results: CD Spectra of MYC, DROSHA, DGCR8, and DICER Oligonucleotides in the Presence of ATP and ADAAD
06:18
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
使用CD光谱描述了ATP依赖性染色质重塑剂与DNA配体的相互作用。通过产生的峰分析的基因启动子上诱导的构象变化可用于了解转录调控的机制。
Tags
CD Spectroscopy
DNA-protein Interactions
ATP-dependent Chromatin Remodeling
Transcription Regulation
NADH Coupled Oxidation Assay
DNA Structure
Oligonucleotides
Quartz Cuvettes
CD Spectra
Baseline Spectra
Experimental Reactions
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