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Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells
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Biochemistry
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JoVE Journal Biochemistry
Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells

Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells

DOI:
10.3791/59519-v

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13:04 min

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May 16, 2019

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, , , , ,
1Department of Biochemistry and Molecular Pharmacology,University of Massachusetts Medical School, 2Department of Chemistry,Skidmore College, 3Candiac MR Center,Beth Israel Deaconess Medical Center, 4Facultad de Ciencias Químico Biológicas,Universidad Autónoma de Guerrero

Chapters

  • 00:04Title
  • 02:51Mammalian Cell Culture Sample Preparation for AAS (Atomic Absorption Spectrometry): Whole Cell and Subcellular Fractionation
  • 05:59Zn Content Analysis of Whole Cell and Subcellular Fractions of Mammalian Cell Cultures by AAS
  • 09:28Results: Purity of Subcellular Fractions and Zinc Levels in Different Mammalian Cultured Cells
  • 11:11Conclusion

Summary

Automatic Translation

Automatic Translation

Cultured primary or established cell lines are commonly used to address fundamental biological and mechanistic questions as an initial approach before using animal models. This protocol describes how to prepare whole cell extracts and subcellular fractions for studies of zinc (Zn) and other trace elements with atomic absorbance spectroscopy.

Tags

Keywords: Atomic Absorbance SpectroscopyIntracellular ZincMammalian CellsTransition MetalsMetal HomeostasisGraphite Furnace Atomic Absorption Spectroscopy (GF-AAS)Trace Element AnalysisMetal MisbalancesPathological ConditionsMethod DevelopmentSample PreparationLow Sample VolumeMethod Validation

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