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JoVE Journal
Biochemistry

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Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells
 

Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells

Article doi: 10.3791/59519
May 16th, 2019

Summary May 16th, 2019

Cultured primary or established cell lines are commonly used to address fundamental biological and mechanistic questions as an initial approach before using animal models. This protocol describes how to prepare whole cell extracts and subcellular fractions for studies of zinc (Zn) and other trace elements with atomic absorbance spectroscopy.

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