Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells

Shellaina  J.V. Gordon; Yao Xiao; Amanda  L. Paskavitz; Napole&#xF3;n Navarro-Tito; Juan  G. Navea; Teresita Padilla-Benavides

doi:10.3791/59519

Journal

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Biochemistry

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Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells

38,429 Views

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13:04 min

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May 16, 2019

DOI:

10.3791/59519-v

DOI:

10.3791/59519-v

•

13:04 min

May 16, 2019

•

10 Views

Shellaina J.V. Gordon*¹, Yao Xiao*², Amanda L. Paskavitz³, Napoleón Navarro-Tito⁴, Juan G. Navea², Teresita Padilla-Benavides¹

¹Department of Biochemistry and Molecular Pharmacology,University of Massachusetts Medical School, ²Department of Chemistry,Skidmore College, ³Candiac MR Center,Beth Israel Deaconess Medical Center, ⁴Facultad de Ciencias Químico Biológicas,Universidad Autónoma de Guerrero

Summary

Cultured primary or established cell lines are commonly used to address fundamental biological and mechanistic questions as an initial approach before using animal models. This protocol describes how to prepare whole cell extracts and subcellular fractions for studies of zinc (Zn) and other trace elements with atomic absorbance spectroscopy.