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Biochemistry

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JoVE Journal Biochemistry
Bacteriële expressie en zuivering van humane matrix metalloproteinase-3 met behulp van affiniteitschromatografie
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Bacteriële expressie en zuivering van humane matrix metalloproteinase-3 met behulp van affiniteitschromatografie

Article DOI: 10.3791/63263-v • 07:32 min • March 30th, 2022
March 30th, 2022 •
*1, *1, 1
1Department of Chemical & Materials Engineering, University of Nevada, Reno
* These authors contributed equally

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His-tag zuivering, dialyse en activering worden gebruikt om de opbrengst van oplosbare, actieve matrix metalloproteinase-3 katalytische domein eiwitexpressie in bacteriën te verhogen. Eiwitfracties worden geanalyseerd via SDS-PAGE gels.

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Biochemie Matrix metalloproteinase MMP-3cd Bacteriële expressie Oplosbaarheid en hervouwen van menselijke eiwitten in E. coli His-tag affiniteit eiwitzuivering.
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