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JoVE Journal Biochemistry
Ekstraktion og oprensning af FAHD1-protein fra svinenyre og muselever
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Ekstraktion og oprensning af FAHD1-protein fra svinenyre og muselever

Article DOI: 10.3791/63333-v • 12:07 min • February 18th, 2022
February 18th, 2022 •
1, 1, 1, 1, 1
1Research Institute for Biomedical Aging Research, University of Innsbruck

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Denne protokol beskriver, hvordan man ekstraherer fumarylacetoacetathydrolasedomæneholdigt protein 1 (FAHD1) fra svinenyre og muselever. De anførte metoder kan tilpasses andre proteiner af interesse og modificeres til andre væv.

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Biokemi udgave 180 FAHD FAHD1 oxaloacetat decarboxylase ODx væv FPLC kromatografi proteinekstraktion
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