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2 i 1: et-trins affinitet rensning for den parallelle analyse af Protein-Protein og Protein-metabolit komplekser
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Chapters
- 00:04Title
- 00:47Preparation of Plant Cell Material
- 01:55Tandem Affinity Purification (TAP)
- 04:59Metabolite and Protein Extraction
- 06:15Results: Simultaneous Characterization of Protein-Protein and Protein-Metabolite Interactions Employing a Single-Step Tandem Affinity Purification Coupled with Mass Spectrometry
- 07:23Conclusion
Protein-protein og protein-metabolit interaktioner er afgørende for alle cellulære funktioner. Heri, beskriver vi en protokol, der tillader parallel analyse af disse interaktioner med et protein valg. Vores protokol var optimeret til plante cellekulturer og kombinerer affinitet rensning med massespektrometri-baseret protein og metabolitten påvisning.
Tags
Affinity PurificationProtein-protein ComplexesProtein-metabolite ComplexesParallel AnalysisBasic ResearchMedicalPharmaceuticalBiotechnologicalSmall Molecular LigandsIn Vivo ConditionsInteracting MoleculesPSBL Arabidopsis ThalianaCell CultureMSMO MediumLogarithmic Growth PhaseMixer MillLysis BufferIgG Sepharose Beads
