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Journal / Biochemistry / Förster Resonance Energy Transfer Mapping: A …
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JoVE Journal Biochemistry
Förster Resonance Energy Transfer Mapping
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Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features

Article DOI: 10.3791/63433-v • 07:09 min • March 16th, 2022
March 16th, 2022 •
1, 1,2, 1,2
1Molecular Biology and Biochemistry Department, Wesleyan University, 2Molecular Biophysics Program, Wesleyan University

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El estudio detalla la metodología del mapeo FRET, incluida la selección de sitios de etiquetado, la elección de tintes, la adquisición y el análisis de datos. Esta metodología es efectiva para determinar los sitios de unión, los cambios conformacionales y los movimientos dinámicos en los sistemas de proteínas y es más útil si se realiza junto con la información estructural 3D existente.

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Bioquímica Número 181 fluorescencia transferencia de energía SecA translocación de proteínas mapeo FRET
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