Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication

Yvonne Gunning; Andrew D. Watson; Neil M. Rigby; Mark Philo; Joshua K. Peazer; E. Kate Kemsley

doi:10.3791/54420

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Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication

JoVE Journal
Biochemistry

This content is Free Access.

JoVE Journal Biochemistry

Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication

11,582 Views

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09:26 min

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September 20, 2016

DOI:

10.3791/54420-v

DOI:

10.3791/54420-v

•

09:26 min

September 20, 2016

•

11560 Views

Yvonne Gunning¹, Andrew D. Watson¹, Neil M. Rigby², Mark Philo¹, Joshua K. Peazer^1,3, E. Kate Kemsley¹

¹Analytical Sciences Unit,Institute of Food Research ²Institute of Food Research ³School of Chemistry,University of East Anglia

Summary

We present a protocol for identifying and quantifying the components in mixtures of species possessing similar proteins. Mass spectrometry detects peptides for identification, and gives relative quantitation by ratios of peak areas. As a tool food for fraud detection, the method can detect 1% horse in beef.