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Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication

Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication

DOI:
10.3791/54420-v

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09:26 min

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September 20, 2016

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, , , , ,
1Analytical Sciences Unit,Institute of Food Research, 2Institute of Food Research, 3School of Chemistry,University of East Anglia

Chapters

  • 00:05Title
  • 00:54Proteolysis and Analysis of Reference Myoglobins
  • 05:14Preparation and Analysis of Calibration Samples
  • 07:57Results: Example Outcomes for a Mixture of Horse in Beef
  • 08:33Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol for identifying and quantifying the components in mixtures of species possessing similar proteins. Mass spectrometry detects peptides for identification, and gives relative quantitation by ratios of peak areas. As a tool food for fraud detection, the method can detect 1% horse in beef.

Tags

Keywords: Species DeterminationQuantitationMeat AuthenticationMRM Mass SpectrometryPeptidesProtein ComponentMyoglobinTrypsin DigestionReversed Phase CartridgeDesaltingVacuum EvaporationPeptide And Transition Prediction Software

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