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Isolatie en functionele analyse van arteriolar endotheel van muishersenparenchym
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Isolatie en functionele analyse van arteriolar endotheel van muishersenparenchym

Article DOI: 10.3791/63463-v • 06:40 min • March 11th, 2022
March 11th, 2022 •
1, 2, 1
1Department of Basic Sciences, Loma Linda University, 2Departments of Physiology, Surgery and Neurosurgery and Sarver Heart Center, University of Arizona College of Medicine Tucson

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Intensieve voorbereiding van intacte cerebrale endotheelbuizen van muizen uit cerebrale parenchymale arteriolen wordt geïllustreerd voor het bestuderen van cerebrale bloedstroomregulatie. Verder demonstreren we de experimentele sterke punten van dit endotheelstudiemodel voor fluorescentiebeeldvorming en elektrofysiologische meting van belangrijke cellulaire signaalroutes, inclusief veranderingen in intracellulaire [Ca2 +] en membraanpotentiaal.

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Biologie hersenmicrocirculatie endotheelhyperpolarisatie Ca2+ signalering K+ kanalen cellulaire beeldvorming elektrofysiologie
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