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小鼠脑实质小动脉内皮的分离及功能分析
JoVE Journal
Biology
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JoVE Journal
Biology
Isolation and Functional Analysis of Arteriolar Endothelium of Mouse Brain Parenchyma
Please note that all translations are automatically generated.
Click here for the English version.
小鼠脑实质小动脉内皮的分离及功能分析
DOI:
10.3791/63463-v
•
06:40 min
•
March 11, 2022
•
Md A. Hakim
,
Paulo W. Pires
,
Erik J. Behringer
1
Department of Basic Sciences
,
Loma Linda University
,
2
Departments of Physiology, Surgery and Neurosurgery and Sarver Heart Center
,
University of Arizona College of Medicine Tucson
Chapters
00:04
Introduction
00:58
Dissection and Isolation of Mouse Cerebral Arterioles
02:30
Digestion of Parenchymal Arterioles and Preparation of Endothelial Tubes
03:47
Utilization of Arteriolar Endothelial Tubes for the Examination of Cellular Physiology and Cellular Imaging
04:54
Results: Assessment of Endothelial Function
05:50
Conclusion
Summary
Automatic Translation
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Automatic Translation
来自脑实质小动脉的完整小鼠脑内皮“管”的强化制备用于研究脑血流调节。此外,我们证明了这种内皮研究模型在荧光成像和关键细胞信号通路的电生理学测量方面的实验优势,包括细胞内[Ca
2 +
]和膜电位的变化。
Tags
Arteriolar Endothelium
Parenchymal Arterioles
Intracerebral Blood Flow
Neuronal And Glial Activity
Morphological Dimensions
Calcium And Electrical Signaling
Endothelial Cells
Enzymatic Digestion
Dissociation Solution
Papain
Dithioerythritol
Collagenase
Elastase
Arteriolar Segments
Endothelial Tubes
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