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Isolering og funktionel analyse af arteriolært endotel af musehjernens parenchyma
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Isolering og funktionel analyse af arteriolært endotel af musehjernens parenchyma

Article DOI: 10.3791/63463-v • 06:40 min • March 11th, 2022
March 11th, 2022 •
1, 2, 1
1Department of Basic Sciences, Loma Linda University, 2Departments of Physiology, Surgery and Neurosurgery and Sarver Heart Center, University of Arizona College of Medicine Tucson

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Intensiv forberedelse af intakte mus cerebrale endotelrør fra cerebrale parenkymale arterioler er illustreret til at studere cerebral blodgennemstrømningsregulering. Desuden demonstrerer vi de eksperimentelle styrker ved denne endotelundersøgelsesmodel til fluorescensbilleddannelse og elektrofysiologisk måling af vigtige cellulære signalveje, herunder ændringer i intracellulær [Ca2+] og membranpotentiale.

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Biologi udgave 181 hjernemikrocirkulation endotelhyperpolarisering Ca2+ signalering K + kanaler cellulær billeddannelse elektrofysiologi
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