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In vivo Positron Emission Tomography to Reveal Activity Patterns Induced by Deep Brain Stimulation in Rats
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1Centro Nacional de Investigaciones Cardiovasculares (CNIC), 2Laboratorio de Imagen Médica,Instituto de Investigación Sanitaria Gregorio Marañón, 3Departamento de Bioingeniería e Ingeniería Aeroespacial,Universidad Carlos III de Madrid, 4CIBER de Salud Mental (CIBERSAM), 5High Performance Research Group in Physiopathology and Pharmacology of the Digestive System (NeuGut),Universidad Rey Juan Carlos
Chapters
- 00:04Introduction
- 00:50Brain Target Localization by In Vivo Neuroimaging
- 02:12Stereotaxic Surgery
- 05:56PET-CT Imaging Acquisition
- 06:46DBS Stimulator Configuration
- 07:30Results: Evaluating the Consequences of Deep Brain Stimulation via In Vivo Neuroimaging
- 08:46Conclusion
We describe a preclinical experimental method to evaluate metabolic neuromodulation induced by acute deep brain stimulation with in vivo FDG-PET. This manuscript includes all experimental steps, from stereotaxic surgery to the application of the stimulation treatment and the acquisition, processing, and analysis of PET images.
