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Isolering av primære retinale retinale pigmenterte epitelceller
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Isolering av primære retinale retinale pigmenterte epitelceller

Article DOI: 10.3791/63543-v • 06:21 min • November 4th, 2022
November 4th, 2022 •
1,2, 3, 1,2, 1,2,3
1Eye Research Institute, Oakland University, 2Eye Research Center (OUWB)/ERC, William Beaumont School of Medicine, 3Oral Biology and Diagnostic Sciences, Dental College of Georgia, Augusta University

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Dette manuskriptet beskriver en forenklet protokoll for isolering av retinale pigmenterte epitelceller (RPE) fra museøyne på en trinnvis måte. Protokollen inkluderer enukleasjon og disseksjon av museøyne, etterfulgt av isolering, såing og dyrking av RPE-celler.

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Biologi utgave 189
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