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Journal
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Biology
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解剖、固定和可视化
果蝇
蛹
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Dissecting, Fixing, and Visualizing the
Drosophila
Pupal Notum
Please note that all translations are automatically generated.
Click here for the English version.
解剖、固定和可视化
果蝇
蛹
DOI:
10.3791/63682-v
•
09:07 min
•
April 06, 2022
•
James S. White
2
,
Kimberly S. LaFever
,
Andrea Page-McCaw
2
1
Dept. Cell and Developmental Biology
,
Vanderbilt University
,
2
Program in Developmental Biology
,
Vanderbilt University
Chapters
00:04
Introduction
00:54
Pupae Immobilization
03:07
Dissecting the Notum
05:59
Mounting the Notum
07:47
Results: Imaging of Notum
08:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本方案详细介绍了果
蝇
蛹固定组织的制备和可视化。它可用于完整或受伤的组织，并保留组织的原始结构。解剖、固定和染色的步骤在本文中均有介绍。
Tags
Keywords: Drosophila
Pupa
Notum
Immunohistochemistry
Dissection
Live-imaging
Antibody Staining
DNA Staining
In Situ Hybridization
Microscopy
Forceps
Adhesive Glue
PBS
Pipette
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