Journal
/
Biology
/
Drosophila Pupal Abdomen Immunohistochemistry
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Drosophila Pupal Abdomen Immunohistochemistry

Drosophila Pupal Abdomen Immunohistochemistry

DOI:
10.3791/3139-v

•

08:29 min

•

October 02, 2011

•

,
1Department of Biological Sciences,University of Alabama

Chapters

  • 00:05Title
  • 01:19Collection, Culturing and Staging Pupae
  • 02:33Dissection
  • 03:40Cleaning, Fixation and Primary Antibody Incubation
  • 05:38Secondary Antibody Incubation and Mounting
  • 07:24Images of Prepared Samples
  • 08:00Conclusion

Summary

Automatic Translation

Automatic Translation

Antibody staining of the Drosophila pupae can enhance genetic analyses of adult abdominal developmental genetics. We present our protocol for dissection, fixation and antibody staining of staged Drosophila pupal abdomen.

Tags

DrosophilaPupal AbdomenImmunohistochemistryEpithelial MorphogenesisSexually Dimorphic MorphologiesImaginal CellsHistoblast NestsLarval EpidermisAdult Abdominal SegmentsDorsal CuticleVentral CuticleSpiraclesDiploid CellsLarval Epidermal CellsMolecular ToolsGenetic PathwaysAdult PhenotypesDissectionImmunohistochemical Analyses

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/3139?article_type=v

Thin Sectioning of Slice Preparations for Immunohistochemistry
check_url/3139?article_type=v

In situ Protocol for Butterfly Pupal Wings Using Riboprobes
check_url/3139?article_type=v

Immunohistochemistry: Paraffin Sections Using the Vectastain ABC Kit from Vector Labs
check_url/3139?article_type=v

Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies
check_url/3139?article_type=v

Drosophila Larval NMJ Immunohistochemistry
check_url/3139?article_type=v

Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas
check_url/3139?article_type=v

Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry
check_url/3139?article_type=v

Imaging Through the Pupal Case of Drosophila melanogaster
check_url/3139?article_type=v

Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment
check_url/3139?article_type=v

Dissection and Mounting of Drosophila Pupal Eye Discs

Read Article