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JoVE Journal
Biology

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Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts
 

Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment

Article doi: 10.3791/51868
September 11th, 2014

Summary September 11th, 2014

This protocol describes the dissection and cultivation of intact testes and germ-line cysts from Drosophila melanogaster pupae. This method allows microscopic observation of spermatogenesis ex vivo. Furthermore, we describe a pharmacological assay of the effect of inhibitors on specific stages of germ-cell development in pupal testes.

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