Dissecting, Fixing, and Visualizing the Drosophila Pupal Notum

DOI:

10.3791/63682-v

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09:07 min

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April 06, 2022

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James S. White², Kimberly S. LaFever, Andrea Page-McCaw²

¹Dept. Cell and Developmental Biology,Vanderbilt University, ²Program in Developmental Biology,Vanderbilt University

Chapters

00:04Introduction
00:54Pupae Immobilization
03:07Dissecting the Notum
05:59Mounting the Notum
07:47Results: Imaging of Notum
08:27Conclusion

Summary

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The present protocol details the preparation and visualization of fixed tissue of the Drosophila pupal notum. It can be used for either intact or wounded tissue, and the original architecture of the tissue is preserved. The procedures for dissecting, fixing, and staining are all described in this article.

Dissecting, Fixing, and Visualizing the Drosophila Pupal Notum

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