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Journal / Immunology and Infection / Microscopía intravital de fluorescencia de campo…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Microscopía intravital de fluorescencia de campo ancho de la microcirculación pulmonar en la lesión pulmonar aguda experimental utilizando un sistema de imágenes estabilizado por vacío
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Microscopía intravital de fluorescencia de campo ancho de la microcirculación pulmonar en la lesión pulmonar aguda experimental utilizando un sistema de imágenes estabilizado por vacío

Article DOI: 10.3791/63733-v • 09:28 min • April 6th, 2022
April 6th, 2022 •
1, 2, 2,3, 4, 5, 6,7, 2,4, 1,2,4,8
1Department of Physiology and Biophysics, Dalhousie University, 2Department of Microbiology and Immunology, Dalhousie University, 3Department of Neuroscience, Dalhousie University, 4Department of Anesthesia, Pain Management, and Perioperative Medicine, Dalhousie University, 5Live Cell Imaging Center, University of Calgary, 6Department of Physiology and Pharmacology, University of Calgary, 7Department of Biochemistry and Molecular Biology, University of Calgary, 8Department of Pharmacology, Dalhousie University

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La microscopía de fluorescencia intravital se puede utilizar para estudiar las interacciones leucocitario-endotelial y la perfusión capilar en tiempo real. Este protocolo describe métodos para obtener imágenes y cuantificar estos parámetros en la microcirculación pulmonar utilizando un sistema de imágenes pulmonares estabilizadas por vacío.

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Inmunología e Infección Número 182
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