00:41Encapsulation of Bacteria with the Photosensitizer
02:07In Situ TEM Observations of Bacterial Cells in Liquid–Electron Dose Effect
02:50In Situ TEM Observations of Light‐Induced Processes Between Bacterial Cells and Photosensitizer
03:51Results: TEM Images of S. aureus Encapsulated with Methylene Blue Between Two Carbon Films and the Unfavorable Effects That May Occur During Imaging
Den nuværende protokol beskriver transmissionselektronmikroskop (TEM) modifikationer med et lysbelysningssystem, fremstilling af flydende celler og in situ TEM-observationer af lysinducerede interaktioner mellem bakterieceller og en fotosensibilisator. Prøveforberedelsesmetoderne, elektronstråleskader og billeddannelse diskuteres også.