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JoVE Journal Engineering
Lysinduceret in situ transmissionselektronmikroskopi til observation af interaktionen mellem flydende og blødt stof
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Lysinduceret in situ transmissionselektronmikroskopi til observation af interaktionen mellem flydende og blødt stof

Article DOI: 10.3791/63742-v • 05:33 min • July 26th, 2022
July 26th, 2022 •
1, 2
1Electron Microscopy Laboratory, Faculty of Mechanical Engineering, Wroclaw University of Science and Technology, 2Advanced Materials Engineering and Modelling Group, Faculty of Chemistry, Wroclaw University of Science and Technology

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Den nuværende protokol beskriver transmissionselektronmikroskop (TEM) modifikationer med et lysbelysningssystem, fremstilling af flydende celler og in situ TEM-observationer af lysinducerede interaktioner mellem bakterieceller og en fotosensibilisator. Prøveforberedelsesmetoderne, elektronstråleskader og billeddannelse diskuteres også.

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