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Produktion af high-titer rekombinant Newcastle Disease Virus fra Allantoic Fluid
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Produktion af high-titer rekombinant Newcastle Disease Virus fra Allantoic Fluid

Article DOI: 10.3791/63817-v • 07:24 min • May 25th, 2022
May 25th, 2022 •
1, 1, 1, 1, 1, 1, 1
1Department of Pathobiology, University of Guelph

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Her giver vi en detaljeret procedure for produktion, rensning og kvantificering af højtitrekombinant Newcastle disease-virus. Denne protokol giver konsekvent > 6 × 109 plaquedannende enheder / ml, hvilket giver virusmængder, der er passende til in vivo dyreforsøg. Yderligere kvalitetskontrol assays for at sikre sikkerhed in vivo er beskrevet.

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Kræftforskning nummer 183
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