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JoVE Journal Cancer Research
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Evaluering cell død ved hjælp af cellefri supernatant af probiotika i tre-dimensionelle sfæroid kulturer colorectal cancerceller

Article DOI: 10.3791/61285-v • 06:07 min • June 13th, 2020
June 13th, 2020 •
*1,2, *3, 1,4,5, 1, 1
1Microbiological Analysis Team, Group for Biometrology, Korea Research Institute of Standards and Science (KRISS), 2College of Pharmacy, Chungnam National University (CNU), 3Stem Cell Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 4Convergent Research Center for Emerging Virus Infection, Korea Research Institute of Chemical Technology (KRICT), 5Department of Bio-Analysis Science, University of Science & Technology (UST)
* These authors contributed equally

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Her metoder præsenteres for at forstå anti-cancer virkninger af Lactobacillus celle-fri supernatant (LCFS). Kolorektal cancer cellelinjer viser celledødsfald, når de behandles med LCFS i 3D kulturer. Processen med at generere sfæroider kan optimeres afhængigt af stilladset, og de fremlagte analysemetoder er nyttige til evaluering af de involverede signalveje.

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Kræftforskning Problem 160 probiotika Lactobacillus fermentum kolorektal cancer sfæroid 3D-kultur Lactobacillus cellefri supernatant (LCFS)
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