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Neuroscience

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JoVE Journal Neuroscience
Hippocampus neuronale kulturer til at detektere og studere nye patogene antistoffer involveret i autoimmun encephalitis
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Hippocampus neuronale kulturer til at detektere og studere nye patogene antistoffer involveret i autoimmun encephalitis

Article DOI: 10.3791/63829-v • 08:20 min • June 2nd, 2022
June 2nd, 2022 •
1, 2, 1, 1,2
1ICFO-Institut de Ciències Fotòniques, The Barcelona Institute of Science and Technology, 2Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Hospital Clínic, Universitat de Barcelona

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Autoimmun encephalitis er en ny kategori af antistofmedierede sygdomme i centralnervesystemet. Hippocampus neuroner kan bruges til at opdage og karakterisere disse antistoffer. Denne artikel indeholder en protokol for primær cellekultur og immunostaining til bestemmelse af autoantistoffer i serum- og cerebrospinalvæsken hos patienter.

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Neurovidenskab udgave 184
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