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JoVE Journal
Bioengineering

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Kemisk triphosphorylation af oligonukleotider
 
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Kemisk triphosphorylation af oligonukleotider

Article DOI: 10.3791/63877-v 13:19 min June 2nd, 2022
June 2nd, 2022

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Summary

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Oligonukleotid 5′-trifosfater er allestedsnærværende komponenter i essentielle biologiske veje og har oplevet stigende anvendelse i bioteknologiske applikationer. Her beskriver vi teknikker til rutinemæssig syntese og oprensning af oligonukleotid-5′-triphosphater, startende fra oligonukleotider fremstillet ved standard automatiserede synteseteknikker.

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Bioengineering udgave 184
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