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Journal / Bioengineering / Diferansiyel Dinamik Mikroskopi Kullanarak…
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Bioengineering

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JoVE Journal Bioengineering
Diferansiyel Dinamik Mikroskopi Kullanarak Sitoiskelet Dinamiğinin Ölçülmesi
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Diferansiyel Dinamik Mikroskopi Kullanarak Sitoiskelet Dinamiğinin Ölçülmesi

Article DOI: 10.3791/63931-v • 06:37 min • June 15th, 2022
June 15th, 2022 •
1, 2, 2, 3, 3, 2, 2
1Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, 2Department of Physics and Biophysics, University of San Diego, 3Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology

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Diferansiyel dinamik mikroskopi (DDM), dinamik ışık saçılması ve mikroskopi özelliklerini birleştirir. Burada, vimentin ağlarındaki parçacıkların subdifüzyon ve kafesli dinamiklerini ve aktif miyozin güdümlü aktin-mikrotübül kompozitlerinin balistik hareketini ölçerek yeniden yapılandırılmış sitoiskelet ağlarını karakterize etmek için DDM'yi kullanma süreci sunulmaktadır.

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