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Bioengineering

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JoVE Journal Bioengineering
Quantificando a Dinâmica do Citoesqueleto usando microscopia dinâmica diferencial
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Quantificando a Dinâmica do Citoesqueleto usando microscopia dinâmica diferencial

Article DOI: 10.3791/63931-v • 06:37 min • June 15th, 2022
June 15th, 2022 •
1, 2, 2, 3, 3, 2, 2
1Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, 2Department of Physics and Biophysics, University of San Diego, 3Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology

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A microscopia dinâmica diferencial (DDM) combina características de dispersão dinâmica de luz e microscopia. Aqui, é apresentado o processo de utilização do DDM para caracterizar redes de citoesqueleto reconstituídas quantificando a dinâmica subdiffusiva e enjaulada de partículas em redes de vimentina e o movimento balístico de compostos ativos de actin-microtubulos movidos a miose.

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Bioengenharia Edição 184
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