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In vitro Modelo enteroide apical de enterocolitis necrosante
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In vitro Modelo enteroide apical de enterocolitis necrosante

Article DOI: 10.3791/64003-v • 09:11 min • June 8th, 2022
June 8th, 2022 •
1, 1, 1
1Department of Pediatrics, Division of Neonatal-Perinatal Medicine, University of Oklahoma Health Sciences Center and Center for Pregnancy and Newborn Health

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Este protocolo describe un modelo apical-out necrotizante enterocolitis (ECN) en un plato que utiliza enteroides del intestino delgado con polaridad invertida, lo que permite el acceso a la superficie apical. Proporcionamos un protocolo de tinción inmunofluorescente para detectar la disrupción epitelial relacionada con NEC y un método para determinar la viabilidad de enteroides apicales sometidos al protocolo NEC en un plato.

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Medicina Número 184
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