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Biochemistry

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JoVE Journal Biochemistry
Un metodo semi-automatico e riproducibile a base biologica per quantificare la deposizione di calcio in vitro
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Journal (Biochemistry)
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Journal (Biochemistry)
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Un metodo semi-automatico e riproducibile a base biologica per quantificare la deposizione di calcio in vitro

Article DOI: 10.3791/64029-v • 11:30 min • June 2nd, 2022
June 2nd, 2022 •
*1, *1, 1, 2, 1, 2, 1,3
1Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, 2Helmholtz Institute for Biomedical Engineering, Biointerface Group, RWTH Aachen University, 3Institute of Experimental Medicine and Systems Biology, RWTH Aachen University
* These authors contributed equally

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Le malattie cardiovascolari sono la principale causa di morte in tutto il mondo. La calcificazione vascolare contribuisce in modo sostanziale al carico di morbilità e mortalità cardiovascolare. Questo protocollo descrive un metodo semplice per quantificare la precipitazione di calcio mediata da cellule muscolari lisce vascolari in vitro mediante imaging fluorescente.

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Biochimica Numero 184
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