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Biochemistry

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JoVE Journal Biochemistry
En semi-automatisert og reproduserbar biologisk-basert metode for å kvantifisere kalsiumavsetning in vitro
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En semi-automatisert og reproduserbar biologisk-basert metode for å kvantifisere kalsiumavsetning in vitro

Article DOI: 10.3791/64029-v • 11:30 min • June 2nd, 2022
June 2nd, 2022 •
*1, *1, 1, 2, 1, 2, 1,3
1Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, 2Helmholtz Institute for Biomedical Engineering, Biointerface Group, RWTH Aachen University, 3Institute of Experimental Medicine and Systems Biology, RWTH Aachen University
* These authors contributed equally

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Hjerte- og karsykdommer er den ledende dødsårsaken over hele verden. Vaskulær forkalkning bidrar vesentlig til byrden av kardiovaskulær sykelighet og dødelighet. Denne protokollen beskriver en enkel metode for å kvantifisere vaskulær glatt muskelcellemediert kalsiumutfelling in vitro ved fluorescerende avbildning.

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