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Karakterisering af neuronalt lysosominteractom med nærhedsmærkning proteomics
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
DOI:

11:40 min

June 23, 2022

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Chapters

  • 00:05Introduction
  • 00:59Proximity Labeling in Human iPSC-Derived Neurons
  • 03:17Enriching Biotinylated Proteins and On-Beads Digestion
  • 06:39LC-MS Analysis
  • 07:15Proteomics Data Analysis
  • 08:36Results: Lysosome Proximity Labeling Proteomics Study Conducted in Human iPSC-Derived Neurons
  • 10:43Conclusion

Summary

Automatic Translation

En neuronal lysosomnærhed mærkning proteomics protokol er beskrevet her for at karakterisere det dynamiske lysosomale mikromiljø i humant inducerede pluripotente stamcelleafledte neuroner. Lysosomale membranproteiner og proteiner, der interagerer med lysosomer (stabilt eller forbigående), kan nøjagtigt kvantificeres i denne metode med fremragende intracellulær rumlig opløsning i levende humane neuroner.

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