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JoVE Journal Neuroscience
Mikrofluidik-unterstützte selektive Depolarisation axonaler Mitochondrien
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Mikrofluidik-unterstützte selektive Depolarisation axonaler Mitochondrien

Article DOI: 10.3791/64196-v • 06:55 min • August 4th, 2022
August 4th, 2022 •
*1,2, *1,2, 2,3,4
1TUM Medical Graduate Center, Technical University of Munich, 2Max Planck Institute of Neurobiology, 3TUM School of Medicine, Institute of Neuronal Cell Biology, Technical University of Munich, 4Munich Cluster for Systems Neurology
* These authors contributed equally

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Das vorliegende Protokoll beschreibt die Aussaat und Färbung neuronaler Mitochondrien in mikrofluidischen Kammern. Der fluidische Druckgradient in diesen Kammern ermöglicht die selektive Behandlung von Mitochondrien in Axonen, um ihre Eigenschaften als Reaktion auf pharmakologische Herausforderungen zu analysieren, ohne das Zellkörperkompartiment zu beeinträchtigen.

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Neurowissenschaften Ausgabe 186 Axon Mitochondrien Mikrofluidik Neurodegeneration
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