Journal
/
Neuroscience
/
Measurement of Oxygen Consumption Rate in Acute Striatal Slices from Adult Mice
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Measurement of Oxygen Consumption Rate in Acute Striatal Slices from Adult Mice

Measurement of Oxygen Consumption Rate in Acute Striatal Slices from Adult Mice

DOI:
10.3791/63379-v

•

07:41 min

•

June 08, 2022

•

, , , , , , , ,
1Department of Neuroscience,Thomas Jefferson University, 2School of Biomedical Engineering,Drexel University, 3Department of Pathology, MitoCare Center,Thomas Jefferson University, 4School of Life Sciences,Peking University

Chapters

  • 00:04Introduction
  • 00:57Hydrating the Cartridge Sensors
  • 01:45Preparing the Tissue Plate
  • 02:24Acute Striatal Slice Preparation and Excised Slice Placement
  • 04:11Loading of Cartridges with Desired Compounds
  • 05:17Calibration and Performing the Assay
  • 06:07Results: Measuring the Oxygen Consumption Rates in Acute Striatal Slices from Adult Mice
  • 07:11Conclusion

Summary

Automatic Translation

Automatic Translation

Oxygen consumption rate (OCR) is a common proxy for mitochondrial function and can be used to study different disease models. We developed a new method using a Seahorse XF analyzer to directly measure the OCR in acute striatal slices from adult mice that is more physiologically relevant than other methods.

Tags

Keywords: Oxygen Consumption RateAcute Striatal SlicesAdult MiceSeahorse XF AnalyzerMitochondrial FunctionParkinson’s DiseaseHuntington’s DiseaseExtracellular Flux AssayArtificial Cerebral Spinal FluidVibratomeCoronal Striatal SlicesRespiration Buffer

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63379?article_type=v

Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology
check_url/63379?article_type=v

Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry
check_url/63379?article_type=v

Time-lapse Imaging of Neuroblast Migration in Acute Slices of the Adult Mouse Forebrain
check_url/63379?article_type=v

Preparation of Acute Subventricular Zone Slices for Calcium Imaging
check_url/63379?article_type=v

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices
check_url/63379?article_type=v

Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators
check_url/63379?article_type=v

Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents
check_url/63379?article_type=v

Preparing Fresh Retinal Slices from Adult Zebrafish for Ex Vivo Imaging Experiments
check_url/63379?article_type=v

Assessment of Long-term Depression Induction in Adult Cerebellar Slices
check_url/63379?article_type=v

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice

Read Article