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Immunology and Infection

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JoVE Journal Immunology and Infection
Separazione delle sottopopolazioni di cellule immunitarie nei campioni di sangue periferico da bambini con mononucleosi infettiva
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Separazione delle sottopopolazioni di cellule immunitarie nei campioni di sangue periferico da bambini con mononucleosi infettiva

Article DOI: 10.3791/64212-v • 08:44 min • September 7th, 2022
September 7th, 2022 •
1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, 2Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences

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Descriviamo un metodo che combina sfere immunomagnetiche e selezione cellulare attivata dalla fluorescenza per isolare e analizzare sottopopolazioni definite di cellule mononucleate del sangue periferico (monociti, cellule T CD4 +, cellule T CD8 +, cellule B e cellule natural killer). Utilizzando questo metodo, le cellule magnetiche e fluorescenti possono essere purificate e analizzate.

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Immunologia e infezione numero 187 virus di Epstein-Barr (EBV) mononucleosi infettiva (IM) selezione di sfere immunomagnetiche selezione di cellule attivate fluorescenti (FACS) sottopopolazioni di cellule immunitarie
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