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Scheiding van immuuncelsubpopulaties in perifere bloedmonsters van kinderen met infectieuze mononucleosis
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis
DOI:

08:44 min

September 07, 2022

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Chapters

  • 00:05Introduction
  • 00:36Isolation of CD14+ Monocytes from PBMCs Using CD14 Microbeads
  • 02:38Fluorescently Labeled Antibody Staining
  • 03:52Flow Cytometry Parameter Setting
  • 04:32Gating
  • 06:18Cell Sorting and Collection via Flow Cytometry
  • 07:00Results: Comparison of Immune Cell Subpopulations from Patients with Infectious Mononucleosis, Healthy Epstein Barr Virus Carriers, and Uninfected Samples
  • 08:14Conclusion

Summary

Automatic Translation

We beschrijven een methode die immunomagnetische kralen en fluorescentie-geactiveerde celsortering combineert om gedefinieerde immuuncelsubpopulaties van perifere mononucleaire bloedcellen (monocyten, CD4 + T-cellen, CD8 + T-cellen, B-cellen en natural killer-cellen) te isoleren en te analyseren. Met behulp van deze methode kunnen magnetische en fluorescerend gelabelde cellen worden gezuiverd en geanalyseerd.

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