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Forming, Confining, and Observing Microtubule-Based Active Nematics
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JoVE Journal Biology
Forming, Confining, and Observing Microtubule-Based Active Nematics

Forming, Confining, and Observing Microtubule-Based Active Nematics

DOI:
10.3791/64287-v

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08:37 min

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January 13, 2023

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, , ,
1Department of Physics,University of California

Chapters

  • 00:04Introduction
  • 01:14Creating the Active Nematic
  • 04:58Inverted Method for Active Nematic Formation
  • 07:26Results: Fluorescence Microscope Image of GMPCPP Microtubules
  • 08:06Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here are methods for preparing active nematics from microtubules and kinesin motors, including protein preparation and construction and the use of wells for active nematic confinement.

Tags

Keywords: MicrotubuleActive NematicsConfined SystemsExperimental TechniquesHydrophilic/hydrophobic SurfacesAcrylamide CoatingFluoro SurfactantMicroscale GeometriesNonlinear DynamicsLiquid CrystalsTopological DefectsChaotic Dynamics

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