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En internt bygget og lysdiodebaseret fotodynamisk terapianordning til forbedring af verteporfincytotoksicitet i en 2D-cellekulturmodel
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model
DOI:

11:04 min

January 13, 2023

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Chapters

  • 00:02Introduction
  • 00:40Device Construction
  • 03:10Cell Lines: Cultivation, Seeding, and Treatment
  • 05:19Device Operation
  • 06:56Cell Viability Assays
  • 07:47Representative Results
  • 09:50Conclusion

Summary

Automatic Translation

Her beskriver vi en ny, enkel og billig enhed til succesfuldt at udføre in vitro fotodynamisk terapi (PDT) assays ved hjælp af todimensionel HeLa-cellekultur og verteporfin som en fotosensibilisator.

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