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从人窦卵泡中提取、标记和纯化谱系特异性细胞
 
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从人窦卵泡中提取、标记和纯化谱系特异性细胞

Article DOI: 10.3791/64402-v 06:36 min November 30th, 2022
November 30th, 2022

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Summary

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在这里,我们提出了从窦卵泡中鉴定和纯化卵巢细胞的方案。我们详细说明了处理整个卵巢以冷冻保存皮质条的方法,同时还收获完整的窦卵泡,这些卵泡经过酶处理以释放多种卵泡常驻细胞类型,包括颗粒细胞、膜细胞、内皮细胞、造血细胞和基质细胞。

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生物学,第189期,
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