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Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
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Chapters
- 00:05Introduction
- 00:51Nocodazole Treatment and Live Imaging
- 02:41Monitoring the Pattern of Securin-GFP Degradation During Meiotic Maturation
- 04:42Recruitment of MAD2 at Kinetochores by Immunofluorescence During Meiotic Maturation
- 07:26Results: Evaluating Spindle Assembly Checkpoint Integrity in Mouse Oocytes
- 09:33Conclusion
Error in chromosome segregation is a common feature in oocytes. Therefore, studying the spindle assembly checkpoint gives important clues about the mechanisms needed to produce healthy eggs. The present protocol describes three complementary assays to evaluate spindle assembly checkpoint integrity in mouse oocytes.
