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Journal / Biochemistry / Imagem de células vivas de globos ex vivo…
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Biochemistry

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JoVE Journal Biochemistry
Imagem de células vivas de globos ex vivo intactos usando um novo suporte impresso em 3D
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Imagem de células vivas de globos ex vivo intactos usando um novo suporte impresso em 3D

Article DOI: 10.3791/64510-v • 05:41 min • October 6th, 2022
October 6th, 2022 •
*1, *2, 2, 3, 2,3
1Department of Pharmacology, School of Medicine, Boston University, 2Department of Biochemistry, School of Medicine, Boston University, 3Department of Ophthalmology, School of Medicine, Boston University
* These authors contributed equally

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O presente trabalho descreve um novo protocolo experimental que utiliza um suporte impresso em 3D para permitir imagens de células vivas de alta resolução de globos enucleados. Através deste protocolo, a atividade de sinalização celular de cálcio no epitélio corneano ferido de globos ex vivo pode ser observada em tempo real.

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Bioquímica Edição 188
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