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Journal / Biochemistry / Preparação de Lamelas a partir de Amostras…
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Biochemistry

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JoVE Journal Biochemistry
Preparação de Lamelas a partir de Amostras Biológicas Vítreas Usando um Microscópio Eletrônico de Varredura de Feixe Duplo para Tomografia Crio-Eletrônica
Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
Journal (Biochemistry)
Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles
Journal (Biochemistry)
Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles
 
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Preparação de Lamelas a partir de Amostras Biológicas Vítreas Usando um Microscópio Eletrônico de Varredura de Feixe Duplo para Tomografia Crio-Eletrônica

Article DOI: 10.3791/62350-v • 07:00 min • August 5th, 2021
August 5th, 2021 •
1,2, 3,4, 3, 1, 1
1Centre for Ultrastructural Imaging, New Hunt’s House, Guy’s Campus, King’s College London, 2Department of Biological Science, Birkbeck College, University of London, 3Electron Bio-Imaging Centre, Diamond Light Source, Harwell Science and Innovation Campus, 4SLAC National Accelerator Laboratory, Stanford University

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Usando fresagem por feixe de íons focalizado para produzir lamelas vítreas on-grid a partir de amostras biológicas congeladas por imersão para tomografia crio-eletrônica.

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Retração FIB-milling cryoFIB-SEM lamela tomografia cryoET cryoEM Plasmodium falciparum
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