Journal
/
/
微生物和化学刺激诱导的中性粒细胞胞外陷阱的形态学和组成分析
JoVE Journal
Immunology and Infection
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli
DOI:

14:05 min

November 04, 2022

, , , , , ,

Chapters

  • 00:05Introduction
  • 01:35Peripheral Blood Collection and Polymorphonuclear Neutrophil Purification
  • 04:11Neutrophil Morphology and Viability
  • 07:28CFSE Staining of Microorganisms and NET Induction
  • 11:10Results: Analysis of NET Induced in Human Blood Derived Neutrophils
  • 13:19Conclusion

Summary

Automatic Translation

这里介绍的是用于体外中性粒细胞细胞 陷阱(NET)的诱导和分析方案。对DNA,cathelicidin(LL37)和酶活性的定量产生了数据,这些数据显示了在类似受控条件下由微生物和化学刺激诱导的NET的组成和形态的变化。

Related Videos

Read Article