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Immunology and Infection
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JoVE Journal Immunology and Infection
Morfologische en compositorische analyse van neutrofiele extracellulaire vallen geïnduceerd door microbiële en chemische stimuli
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Morfologische en compositorische analyse van neutrofiele extracellulaire vallen geïnduceerd door microbiële en chemische stimuli

Article DOI: 10.3791/64522-v • 14:05 min • November 4th, 2022
November 4th, 2022 •
1, 2, 1, 3, 4, 3, 1
1Clinical Immunology Research Department of Biochemical Sciences Faculty, Universidad Autónoma “Benito Juárez” de Oaxaca, 2Department of Molecular Biomedicine, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, 3CONACYT- Medicine and Surgery Faculty, Universidad Autónoma “Benito Juárez” de Oaxaca, 4Molecular Immunology Research Department of Medicine and Surgery Faculty, Universidad Autónoma “Benito Juárez” de Oaxaca

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Hier wordt een protocol gepresenteerd voor de inductie en analyse van in vitro neutrofiele extracellulaire vallen (NET's). Kwantificering van DNA, cathelicidin (LL37) en enzymactiviteit leverde gegevens op die de variabiliteit in de samenstelling en morfologie van NET's geïnduceerd door microbiële en chemische stimuli onder vergelijkbare gecontroleerde omstandigheden aantonen.

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Immunologie en infectie nummer 189
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